I have a nextcloud instance being hosted from my home network. The URL associated with it points directly at my home's IP. I don't want to host the instance on a VPS because disk space is expensive. So, instead, I want to point the URL at the VPS, and then somehow route the connection to my home's nextcloud instance without leaking my home's ip.
How might I go about doing this? Can this be achieved with nginx?
EDIT: Actually, not leaking my home's IP is not essential. It is acceptable if it is possible to determine the IP with some effort. What I really want is to be able to host multiple websites with my single home IP without those websites being obviously connected, and to avoid automatic bots constantly looking for vulnerabilities in my home network.
Good luck! I look forward to your healthy grain spawn pics!
Ooh wow, yeah that's a big pressure canner! I work with a smaller pressure cooker - the kind used for preparing meals (a 6L and a 10L, 12 PSI). You probably don't want to use such a big canner for cooking the grains. I would just boil them them in a regular pot instead.
Usually the procedure is separated into the 'hydrating' step and then the 'sterilization' step, because this way you can ensure that the grain is hydrated to the right amount, that it is evenly hydrated, and that the surface is dry. It is possible to mix the dry grains and the right amount of water inside of the jar and then just let the grains hydrate while you pressure cook them. Some people online say that they use this method, but I did not get great results when I tried it.
For the sterilization you really should use that pressure canner. Placing the jars inside the canner and running it at 15 PSI for 90 minutes (after venting for 10 - 15 min) is enough to sterilize grain.
But what you have is indeed a big canner... If you don't want to use it, you can try to simply sterilize by boiling both the grain and the jar. In that case I would not worry about drying the grain at all - the grain would go straight into the jar while both are still hot. Then you have a reasonable chance of not having contaminants. With this procedure you might have grains that are too wet and heat-resistant endospores of bacteria that can survive boiling, so you may get bacterial contamination. But is not a certainty - many times it works fine. Do what's simplest, and if your contamination rate is above the level that you find acceptable then you can take steps to improve your process.
I do add some gypsum some times, and sometimes I don't add it. I think the main benefit is nutritional in that it adds some calcium. It can help dry out the surface of the grains a bit if you add it after cooking the grain, but it only helps to a degree - if your grains are over-hydrated or too wet, gypsum will not be enough to stop them from becoming a hardened block.
As for pressure cooking the grain - this is done with the grains directly in water with more than enough water to cover them. I use the pressure cooker in this step just because it is faster and more efficient. The rate at which grains absorb water is proportional to the temperature, and the liquid water gets hotter in the pressure cooker due to the increase in boiling point at the higher pressure. This rate depends on the pressure achieved by your pressure cooker (usually 12 PSI or 15 PSI), and a bit in the altitude. That's why the best is to test the hydration with your setup instead of relying on a set recipe.
There is no need to protect the kernels while they dry. The sterilization step comes after, once the grain jars are prepared. In theory you can get unlucky and an heat-resistant endospore could land into the drying grain, so you might want to be a bit clean. I personally I just let it dry by the sink. Get them out as soon as possible while they are hot because this helps the water on the surface evaporate quicker. You can even use a towel to dry them but I find that rubbing grains with a towel is a bit awkward.
So this morning’s viewing of my cremini spawn… whatever is growing in there is definitely expanding.
That's great! Exciting :D Good luck!
Yeah! Look, here I have two jars of popcorn kernel grain spawn. On each one of the jars I put in a small piece of a King Oyster mushroom that I bought in the supermarket. I rub 70% isopropanol around the mushroom and then I tear the mushroom open and take out a tiny piece of issue from the inside. These jars were inoculated on May 16, so this is 23 days of growth:
The left one I already shook and the right one is still un-shaken.
What I mean about the hydration is that with rye grain I would often end up with grains a bit too wet and sticky, making it impossible to shake the spawn properly. The colonized grain would turn into a hard block that I had to take out with a spoon! With properly hydrated and surface-dried grain, you can shake the grains and they separate very well, making it much easier to work with them.
What I do is I buy one of these bags from the supermarket (900 g, cost is about $2.5 per kilo or $1.13 per pound):
No need to pre-soak them. I cook them in the pressure cooker for 25 - 30 minutes, starting the timer once pressure is reached without venting (this is not a sterilization step, just a cooking step). Without a pressure cooker they can be boiled for about 1 hour. Then I place them in a colander to dry - at least one hour, over night is even better. The grains should not be wet outside and they should still be firm, but it should be possible to crush them between your fingers if a moderate amount of force is applied.
Since each person has a different setup, the best way to get the right hydration for your own setup is to measure the amount of water absorbed a few times until you are happy with your procedure. You weight the grains before cooking them, and you weight them again after cooking and drying them. The increase in weight for popcorn of 40% - 70% would be a good amount of hydration.
$17 for 4 pounds of rye is quite expensive... That comes out to 4.25 / lb! Current prices I can find for my region are closer to $0.5 / lb for buying bulk. So for a mushroom farmer that buys rye in bulk from as close to the source as possible it makes a lot of sense to use rye because it is a really good grain and cheap, but when it is not sold locally and you have to order it from a specialty shop you are paying a high premium for it! Hope that makes sense!
Cool! Good luck!! It is super fun :-)
I know that rye grain is often recommended - and it makes sense for commercial growers who can get lots of cheap rye grain and who have mastered the process of hydrating and sterilizing rye. But I have personally moved back to popcorn kernels... I can buy them at my local shop, it is easy to hydrate them to the right level, and I have less contamination. I mention this because I read that you are "waiting to receive some rye grain" - which sounds like you may have ordered it online! Consider that you can get similar or even better success with the grains that are readily available.
If you have any doubts about contamination you can post some photos and I might be able to help!
I am trying to grow Oakmoss lichen (Evernia prunastri) at home, which is a highly valued lichen in the perfume industry due to its woody and sweet odor.
Lichens consists of an association between at least a fungus and a green algal partner - but often other organisms like yeasts and cyanobacteria are also present in the association.
I tore some oakmoss to expose the white tissue underneath, and you can see the fungal hyphae and the algae:
To culture a fungus one usually begins by isolating it away from the many contaminants that are naturally present on the fungus. Lichens are more problematic because in their lichenized form they grow too slowly to succesfully out-pace contaminants. The organisms that they are made of grow faster when they are not lichenized, but they have different methods of isolation. The trick to grow them is to isolate the fungus (mycobiont) and the alga (photobiont) separately and then try to bring them together in a process called "resynthesis".
I am trying to do this process at home, starting from a small piece of lichen that I collected from the bark of a tree. This is what grew out of the tissue sample when it first went into the agar, showing lots of contaminants fighting for the nutrients in the dish:
And this is the growth after the first transfer:
In parallel, I am trying to isolate the algal partner, which is a Trebouxia. One method is to place the tissue in agar plates with no nutrients and expose the plate to the sun. The other method is to place the tissue in a solution of orchid fertilizer in water and leave it by the window.
The cells of the alga are easily released into the water when the lichen is crushed. I confirmed that under the microscope. This is a photo of the released Trebouxia cells under the microscope:
The plate in the image associated with this post is a plate of the Evernia mycobiont that had bacterial contamination in it. Rather than throwing it away, I decided to crush some lichenized tissue in water and pour in on top of the lichen to check whether that is enough to achieve lichenization.
Just wanted to share some of my experiments! Any input on how to achieve the resynthesis would be greatly appreciated ;-)