I disagree. Seeing the troll character standing there still smiling as if nothing has happened, and the AAAAAAAAH that has completely replaced the trolled character really punctuates it, imo.
Dark souls isn't the only game with a dodge. In fact, in dark souls traditionally just pressing dodge will get you a back step, not a roll, so I'd argue that this isn't specifically a dark souls reference, just action RPGs in general.
It's okay that it's not from dark souls. The joke still works, some games use X to dodge, and some of them include multiplayer and narrow platforms with death below.
When I booted up Final Fantasy VII for the first time on my PS1, I was so confused as to why I could not start a new game. I was young, but I'm pretty sure I just shrugged and put the game away for months.