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InitialsDiceBearhttps://github.com/dicebear/dicebearhttps://creativecommons.org/publicdomain/zero/1.0/„Initials” (https://github.com/dicebear/dicebear) by „DiceBear”, licensed under „CC0 1.0” (https://creativecommons.org/publicdomain/zero/1.0/)PH
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  • Die Murderbot Diaries fangen in der Dystopie an und werden schrittweise hoffnungsvoller (Mit Höhen und Tiefen). Murderbot selbst verschiebt die eigenen Vorstellungen und Werte im Verlauf der Serie mehr hin zu dem, was wir als "normal" und eben nicht als dystopisch empfinden (ohne das gerne zugeben zu wollen).

  • So ... we should've hanged all the Germans in '45? The child soldiers drafted in Berlin? Shot the entire Japanese army? Drag all US-citizens to court for acting against the constitution?

    I am going to be downvoted to oblivion, but: Lock up all Palestinians for not extraditing terrorists and war criminals?

    Where do you think this will go, honestly? How is any of that justified, or fair? Should we commit a genocide of our own upon the Israelis? I know that's not what you said and I very much hope it's not what you meant.

    This platform sometimes, fuck me.

  • Etwas naive interessensfrage: Haben die irgendwelche Dienstausweise (bzw. deren Mangel) oder dergleichen, mit denen man sie entlarven kann? Müssen die angeben, für wen sie arbeiten?

  • Permanently Deleted

    Jump
  • Ich verstehe den Hintergrund nicht ganz, finde die stereotypische Darstellung von Bayern als Fieslinge aber etwas schwierig. Bloß mein (süßer Weißwurst) Senf, mach daraus was du willst.

  • To produce ABs in cell culture you usually create a hybridoma cell line, by fusing the B-Cell to an immortal tumour cell line (as you postulated). This approach does however require you to identify the cells that produce the proper antibody.

    It gets much easier if you know the exact sequence/structure. To learn this (or find a better one) it is also possible to generate libraries of antibody sequences with semi-random hypervariable regions (the part that binds the antibody) using for example phages that will display any protein on their surface area and then measuring the binding affinity! This is called - drum roll - phage display (very creative).

    Alright, enough trivia for now.