2w ago

CRISPR-Cas9

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Explanation: CRISPR-Cas9 is most commonly known to be a gene editor, but since its initial discovery, people have found that with some minor tweaks, CRISPR can be made to do a bunch of other things, things that biologists could have only dreamed of previously.
If you introduce a mutation that deactivates the Cas9 protein, the entire CRISPR complex still binds to DNA. Then, you can essentially glue whatever you want onto this modified Cas9 to produce a bunch of different effects:
deactivated Cas9, no additional modifications: blocks other proteins from touching the DNA, which suppresses a gene without destroying it
glue on some transcriptional activators: makes the cell express a gene
glue on some fluorescent proteins: makes your DNA glow, lets you see where it is
- Well done! I zoomed in and felt dumb. But thanks to your succinct explanation, I now have a sweet Dunning-Kruger high
Genetic engineering has never been this cute
- I dunno. For hundreds of thousands of years we've been selectively breeding puppies.
  Puppies
  
  In a way one could argue genetic engineering is one of the oldest sciences ever. And it started with dogs, or maybe agriculture, depends on what one's definition of science is.
  
  Whatever we did to pugs is definitely not cute. Or okay.
I want to >!fuck!< CRISPRi
- Your spoiler failed
  
  Your ~~spoiler~~ transcription repressor failed
  FTFY 👍
  
  Sadly there are two spoiler types i know of :/
  for me the spoiler works because i use the "second one?"
- "Did you know..."
  
  "...that in terms of male human and female CRISPR-Cas9 variant reproduction, CRISPRi is the most compatible CRISPR-Cas9 variant for humans? Not only are they in the genetic perturbation group which is mostly comprised of various methods, CRISPRi are an average of..."
- Can't help it, it's most compatible
- I prefer meowscarada.
  
  Y'all need Jesus. And an assigned FBI agent.

17 comments